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Last Update: 05/24/2024 07:27 PM

Current Deck: MCBL 121::Chapters 6 and 11

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Culturing Bacteriophages - Plaque Assay of Viral Titre

Purpose: to determine the {{c1::titer (PFU/mL) of the original stock solution Bacteriophages are grown in bacteria }}

Bacteriophages form {{c1::plaques}}, which are clearings on a lawn of bacteria on the surface of agar
Each plaque corresponds to a single {{c1::virus}}; can be expressed as plaque-forming units (PFU)